Supporting data for "De Novo PacBio long-read and phased avian genome assemblies correct and add to reference genes generated with intermediate and short reads"
Dataset type: Genomic, Transcriptomic
Data released on August 28, 2017
Korlach J; Gedman G; Kingan S; Chin CS; Howard JT; Audet JN; Cantin L; Jarvis ED (2017): Supporting data for "De Novo PacBio long-read and phased avian genome assemblies correct and add to reference genes generated with intermediate and short reads" GigaScience Database. http://dx.doi.org/10.5524/100311
Reference quality genomes provide a resource for studying gene structure, function, and evolution. However, often genes of interest are not completely or accurately assembled, leading to unknown errors in analyses or additional cloning efforts for the correct sequences. A promising solution is long-read sequencing. Here we tested PacBio-based long-read sequencing and diploid assembly for potential improvements to the Sanger-based intermediate-read zebra finch reference and Illumina-based short-read Anna’s hummingbird reference, two vocal learning avian species widely studied in neuroscience and genomics. With DNA of the same individuals used to generate the reference genomes, we generated diploid assemblies with the FALCON-Unzip assembler, resulting in contigs with no gaps in the megabase range, representing 150-fold and 200-fold improvements over the current zebra finch and hummingbird references, respectively. These long-read and phased assemblies corrected and resolved what we discovered to be numerous misassemblies in the references, including missing sequences in gaps, erroneous sequences flanking gaps, base call errors in difficult to sequence regions, complex repeat structure errors, and allelic differences between the two haplotypes. These improvements were validated by single long genome and transcriptome reads, and resulted for the first time in completely resolved protein-coding genes widely studied in neuroscience and specialized in vocal learning species. These findings demonstrate the impact of long reads, sequencing of previously difficult-to-sequence regions, and phasing of haplotypes on generating high quality assemblies necessary for understanding gene structure, function, and evolution.
Additional details
Read the peer-reviewed publication(s):
(PubMed: 29020750)
Accessions (data generated as part of this study):
BioProject:
PRJNA368994
BioProject:
PRJNA289277
Projects:
Sample ID | Taxonomic ID | Common Name | Genbank Name | Scientific Name | Sample Attributes |
---|---|---|---|---|---|
ChIP-Seq/RA_1 | 59729 | Zebrafinch | Zebrafinch | Description:Chromatin extracted from RA of Zebra f... Alternative names:S-JL091- left leg band # Analyte type:Chromatin ... + | |
ChIP-Seq/RA_2 | 59729 | Zebrafinch | Zebrafinch | Description:Chromatin extracted from RA of Zebra f... Alternative names:S-JL092- left leg band # Analyte type:Chromatin ... + | |
ChIP-Seq/RA_3 | 59729 | Zebrafinch | Zebrafinch | Description:Chromatin extracted from RA of Zebra f... Alternative names:S-JL093- left leg band # Analyte type:Chromatin ... + | |
RNA-Seq/RA_rep1 | 59729 | Zebrafinch | Zebrafinch | Description:RNA extracted from RA of Zebra Finch B... Alternative names:E-JL181 and V-JL246 Analyte type:RNA ... + | |
RNA-Seq/RA_rep2 | 59729 | Zebrafinch | Zebrafinch | Description:RNA extracted from RA of Zebra Finch B... Alternative names:R-JL134 and R-JL310 Analyte type:RNA ... + | |
RNA-Seq/RA_rep3 | 59729 | Zebrafinch | Zebrafinch | Description:RNA extracted from RA of Zebra Finch B... Alternative names:V-JL97 and P-JL1290 Analyte type:RNA ... + | |
RNA-Seq/RA_rep4 | 59729 | Zebrafinch | Zebrafinch | Description:RNA extracted from RA of Zebra Finch B... Alternative names:P-JL1246 and G-JL210 Analyte type:RNA ... + | |
RNA-Seq/RA_rep5 | 59729 | Zebrafinch | Zebrafinch | Description:RNA extracted from RA of Zebra Finch B... Alternative names:no_band Analyte type:RNA ... + | |
SAMN02265252 | 9244 | Annas hummingbird | Annas hummingbird | Calypte anna | Description:DNA extracted from blood and liver of ... Alternative names:BGI_N300 Analyte type:DNA ... + |
SRS1954332 | 59729 | Zebrafinch | Zebrafinch | Description:DNA extracted from /muscle of adult/ m... Alternative names:black17 - leg band # Analyte type:DNA ... + |