Supporting data for "Genome assembly and transcriptome resource for river buffalo, Bubalus bubalis (2n=50)"
Dataset type: Genomic, Transcriptomic
Data released on August 23, 2017
Biagini T; Bomba L; Capomaccio S; Castiglioni B; Coletta A; Corrado F; Ferre F; Iamartino D; Iannuzzi L; Low WY; L Smith TP; Pruitt KD; Sonstegard TS; Williams JL; Lawley C; Macciotta N; McClure M; Mancini G; Matassino D; Mazza R; Milanesi M; Moioli B; Morandi N; Ramunno L; Peretti V; Pilla F; Ramelli P; Schroeder S; Strozzi F; Thibaud-Nissen F; Zicarelli L; Ajmone-Marsan P; Valentini A; Chillemi G; Zimin A (2017): Supporting data for "Genome assembly and transcriptome resource for river buffalo, Bubalus bubalis (2n=50)" GigaScience Database. http://dx.doi.org/10.5524/100333
Water buffalo is a globally important species for agriculture and local economies. A de novo assembled, well annotated, reference sequence for the water buffalo is an important prerequisite for studying the biology of this species, and necessary to manage genetic diversity and to use modern breeding and genomic selection techniques. However, no such genome assembly has been previously reported. There are two species of domestic water buffalo, the river (2n=50) and the swamp (2n=48) buffalo. Here we describe a draft quality reference sequence for the river buffalo created from Illumina GA and Roche 454 short read sequences using the MaSuRCA assembler. The assembled sequence is 2.83 Gb, consisting of 366,983 scaffolds with a scaffold N50 of 1.41 Mb and contig N50 of 21,398 bp. Annotation of the genome was supported by transcriptome data from 30 tissues, and identified 21,711 predicted protein coding genes. Searches for complete mammalian BUSCO gene groups found 98.6% of curated single copy orthologs present among predicted genes, which suggests a high level of completeness of the genome. The annotated sequence is available from NCBI at accession GCA_000471725.1.
Read the peer-reviewed publication(s):
Williams, J. L., Iamartino, D., Pruitt, K. D., Sonstegard, T., Smith, T. P. L., Low, W. Y., … Zimin, A. (2017). Genome assembly and transcriptome resource for river buffalo, Bubalus bubalis (2n = 50). GigaScience, 6(10), 1–6. doi:10.1093/gigascience/gix088
Accessions (data included in GigaDB):
|Sample ID||Taxonomic ID||Common Name||Genbank Name||Scientific Name||Sample Attributes|
|white_blood_cells||89462||domestic water buffalo||water buffalo||Bubalus bubalis|| Description:RNA extracted from snap frozen tissue ...|
Geographic location (latitude and longitude):45.30...
... Description:RNA extracted from snap frozen tissue collected post mortum from 12 month male Italian Mediterranean buffalo calf
Geographic location (latitude and longitude):45.304542, 9.476251
Geographic location (country and/or sea,region):Lombardy, Italy
Life stage:calf [FOODON:03411349]
Common name:water buffalo
Funding source:Italian Consortium
Tissue:white blood cells
Sample source:University of Milan
Sample contact:Daniela Iamartino
Collected by:John Williams and colleagues
Alternative accession-SRA Sample:ERS327324